Escherichia coli Contamination Levels of Water from Unprotected Wells in Chaona Community, Mwachisompola Area of Chibombo District of Zambia
Keywords:
Escherichia coli, Contamination, Unprotected wells, Water
Abstract
A quantitative cross-sectional study was conducted to detect the presence of E. coli in unprotected water wells of Chaona Community in Mwachisompola area, Chibombo District of Zambia. 48 wells drawn from four villages were sampled from the study area. Laboratory processes of culturing, isolation and identification of E. coli confirmed the occurrence of the bacterium in well water. The identified E. coli was subjected to antibiotic resistance testing, and PCR was used to detect the resistant genes further. Of the 48 unprotected wells sampled, 38 (79%, 95% CI: 77.3 – 80.7%) were found contaminated with E. coli. Meanwhile, 16/48 (33.3%; CI: 31.4 – 35.2%) samples were found with an average CFU of between 1000 and 10,000, the highest range. E. coli isolates were also tested for Multi-Drug Resistance (MDR), of which one isolate indicated being resistant to eight antibiotics and another to five antibiotics presenting (5.88%; CI: 3.2 – 8.6%) for each. Meanwhile, seven isolates were resistant to four antibiotics (41.2%; CI: 35.5 – 46.9%), and eight isolates were resistant to three antibiotics (41.1%, CI: 35.4 – 46.9%). In addition, 30.9% (17/55) of the isolated E.coli organisms were found to be resistant to three or more classes of antibiotics primarily; Ampicillin, Streptomycin, Tetracycline, Cefotaxime, Nalidixic Acid, Norfloxacin, and Ciprofloxacin. The study revealed that E. coli contamination was highly possible, and it is recommended that water should be boiled and or treated with chlorine before use at the household level.References
1. Percival SL, Williams DW. Escherichia coli. Microbiology of Waterborne Diseases: Microbiological Aspects and Risks: Second Edition. 2013:89–117.https://doi.org/10.1016/B978-0-12-415846-7.00006-8.
2. Foster NE. The Microbial Ecology of Escherichia coli in the Vertebrate gut. February. 2022:1–22. https://doi.org/10.1093/femsre/fuac008.
3. Ercumen A, Pickering AJ, Kwong LH, Arnold BF, Masud Parvez S, Alam M, et al. Animal Feces Contribute to Domestic Fecal Contamination: Evidence from E. coli Measured in Water, Hands, Food, Flies, and Soil in Bangladesh. 2017. https://doi.org/10.1021/acs.est.7b01710.
4. Gwimbi P, George M, Ramphalile M. Bacterial contamination of drinking water sources in rural villages of Mohale Basin, Lesotho: exposures through neighbourhood sanitation and hygiene practices. Environmental Health and Preventive Medicine. 2019; 24:1-7.
5. Pal M, Ayele Y, Hadush M, Panigrahi S, Jadhav V.J. Public health hazards due to unsafe drinking water. Air Water Borne Dis. 2018; 7(1000138):2.
6. Babuji P, Thirumalaisamy S, Duraisamy K, Periyasamy G. Human Health Risks due to Exposure to Water Pollution: A Review. Water (Switzerland). 2023; 15(14):1–15. https://doi.org/10.3390/w15142532.
7. Bain R, Johnston R, Slaymaker T. Drinking water quality and the SDGs. npj Clean Water. 2020;3 (1):37. https://www.nature.com/articles/s41545-020-00085-z.pdf.
8. UNICEF. 2015 Update and MDG Assessment. UNICEF. 2015.
9. Palaniappan RUM, Zhang Y, Chiu D, Torres A, DebRoy C, Whittam TS, et al. Differentiation of Escherichia coli pathotypes by oligonucleotide spotted array. Journal of Clinical Microbiology. 2006; 44(4):1495–1501. https://doi.org/10.1128/JCM.44.4.1495-1501.2006.
10. Charan J, Biswas T. How to calculate sample size for different study designs in medical research? Indian Journal of Psychological Medicine. 2013; 35(2):121–126.https://doi.org/10.4103/0253-7176.116232.
11. Blount ZD, Barrick JE, Davidson CJ, Lenski RE. Genomic Analysis of a Key Innovation in an Experimental E. coli Population HHS Public Access. Nature. 2012; 489(7417):513–518. https://doi.org/10.1038/nature11514.Genomic.
12. Rehm HL, Bale SJ, Bayrak-Toydemir P, Berg JS, Brown KK, Deignan JL, et al. ACMG clinical laboratory standards for next-generation sequencing. Genetics in Medicine. 2013; 15(9):733–747. https://doi.org/10.1038/gim.2013.92.
13. Begum YA, Talukder KA, Nair GB, Qadri F, Sack RB, Svennerholm AM. Enterotoxigenic Escherichia coli isolated from surface water in urban and rural areas of Bangladesh. Journal of Clinical Microbiology. 2005; 43(7):3582. DOI: 10.1128/JCM.43.7.3582.
14. Phiri A. Risks of Domestic Underground Water Sources in Informal Settlement in Kabwe – Zambia. 2016; 5(2):1–14. DOI: 10.5539/ep.v5n2p1.
15. Chishimba K. Extended spectrum beta-lactamase (ESBL) Producing Escherichia coli in poultry and water in Lusaka District. The University of Zambia; 2015.
16. Larson A, Hartinger SM, Riveros M, Salmon-mulanovich G, Hattendorf J, Verastegui H, Huaylinos ML, Daniel M. Antibiotic-Resistant Escherichia coli in Drinking Water Samples from Rural Andean Households in Cajamarca, Peru. 2019; 100(6):1363–1368. DOI: 10.4269/ajtmh.18-0776.
17. WHO. Water safety plan: a field guide to improving. World Health Organisation; 2014.
18. Thani TS, Symekher SML, Boga H, Oundo J. Isolation and characterisation of Escherichia colipathotypes and factors associated with well and boreholes water contamination in Mombasa county. Pan African Medical Journal. 2016; 23. DOI: 10. 11604/pamj.2016.23.12.7755.
2. Foster NE. The Microbial Ecology of Escherichia coli in the Vertebrate gut. February. 2022:1–22. https://doi.org/10.1093/femsre/fuac008.
3. Ercumen A, Pickering AJ, Kwong LH, Arnold BF, Masud Parvez S, Alam M, et al. Animal Feces Contribute to Domestic Fecal Contamination: Evidence from E. coli Measured in Water, Hands, Food, Flies, and Soil in Bangladesh. 2017. https://doi.org/10.1021/acs.est.7b01710.
4. Gwimbi P, George M, Ramphalile M. Bacterial contamination of drinking water sources in rural villages of Mohale Basin, Lesotho: exposures through neighbourhood sanitation and hygiene practices. Environmental Health and Preventive Medicine. 2019; 24:1-7.
5. Pal M, Ayele Y, Hadush M, Panigrahi S, Jadhav V.J. Public health hazards due to unsafe drinking water. Air Water Borne Dis. 2018; 7(1000138):2.
6. Babuji P, Thirumalaisamy S, Duraisamy K, Periyasamy G. Human Health Risks due to Exposure to Water Pollution: A Review. Water (Switzerland). 2023; 15(14):1–15. https://doi.org/10.3390/w15142532.
7. Bain R, Johnston R, Slaymaker T. Drinking water quality and the SDGs. npj Clean Water. 2020;3 (1):37. https://www.nature.com/articles/s41545-020-00085-z.pdf.
8. UNICEF. 2015 Update and MDG Assessment. UNICEF. 2015.
9. Palaniappan RUM, Zhang Y, Chiu D, Torres A, DebRoy C, Whittam TS, et al. Differentiation of Escherichia coli pathotypes by oligonucleotide spotted array. Journal of Clinical Microbiology. 2006; 44(4):1495–1501. https://doi.org/10.1128/JCM.44.4.1495-1501.2006.
10. Charan J, Biswas T. How to calculate sample size for different study designs in medical research? Indian Journal of Psychological Medicine. 2013; 35(2):121–126.https://doi.org/10.4103/0253-7176.116232.
11. Blount ZD, Barrick JE, Davidson CJ, Lenski RE. Genomic Analysis of a Key Innovation in an Experimental E. coli Population HHS Public Access. Nature. 2012; 489(7417):513–518. https://doi.org/10.1038/nature11514.Genomic.
12. Rehm HL, Bale SJ, Bayrak-Toydemir P, Berg JS, Brown KK, Deignan JL, et al. ACMG clinical laboratory standards for next-generation sequencing. Genetics in Medicine. 2013; 15(9):733–747. https://doi.org/10.1038/gim.2013.92.
13. Begum YA, Talukder KA, Nair GB, Qadri F, Sack RB, Svennerholm AM. Enterotoxigenic Escherichia coli isolated from surface water in urban and rural areas of Bangladesh. Journal of Clinical Microbiology. 2005; 43(7):3582. DOI: 10.1128/JCM.43.7.3582.
14. Phiri A. Risks of Domestic Underground Water Sources in Informal Settlement in Kabwe – Zambia. 2016; 5(2):1–14. DOI: 10.5539/ep.v5n2p1.
15. Chishimba K. Extended spectrum beta-lactamase (ESBL) Producing Escherichia coli in poultry and water in Lusaka District. The University of Zambia; 2015.
16. Larson A, Hartinger SM, Riveros M, Salmon-mulanovich G, Hattendorf J, Verastegui H, Huaylinos ML, Daniel M. Antibiotic-Resistant Escherichia coli in Drinking Water Samples from Rural Andean Households in Cajamarca, Peru. 2019; 100(6):1363–1368. DOI: 10.4269/ajtmh.18-0776.
17. WHO. Water safety plan: a field guide to improving. World Health Organisation; 2014.
18. Thani TS, Symekher SML, Boga H, Oundo J. Isolation and characterisation of Escherichia colipathotypes and factors associated with well and boreholes water contamination in Mombasa county. Pan African Medical Journal. 2016; 23. DOI: 10. 11604/pamj.2016.23.12.7755.
Published
2024-07-05
How to Cite
1.
Zgambo D, Munyeme M, Kabwali E, Thendji L, Hang’ombe B. Escherichia coli Contamination Levels of Water from Unprotected Wells in Chaona Community, Mwachisompola Area of Chibombo District of Zambia. Journal of Agricultural and Biomedical Sciences [Internet]. 5Jul.2024 [cited 4Aug.2025];7(4). Available from: https://journals.unza.zm/index.php/JABS/article/view/1220
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General
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